The study was conductedto assess theeffectiveness of Self Instructional Module (SIM) on knowledge and skill regarding prevention ofHIV/AIDS amongGeneral Nursing and Midwifery students of Ancillary Medical Training School ,Shireenbagh, Kashmir.Methodology:-Aquantitativeapproachwithpre-experimentalonegrouppre-test post-test design was used to conduct the study. Sample comprised60General Nursing and Midwiferyfulfilling the Inclusion criteria by using simple random sampling technique.Data was collected using structured questionnaireto assess the knowledge regarding prevention of HIV/AIDS and Observational checklist to assess skill onPersonal protective equipments. Pre-test was conducted to assess the existing knowledge and skill of the study subjects by structured Questionnaire and observational checklist followed bySelf Instructional Module (SIM)and demonstration .Post test was conducted on 7th day by using same structured. Questionnaire and observational checklist. The data collected were analysed by descriptive & inferential statistics. Results:Out of 60 subjects majority(78.3%) were in theage group of above 20 years , (66.7%)werefromrural area,38 .(63.3%)were from nuclear family, (40.0%)were secondary parental education.Overallmean post-test knowledge score (28.38±3.46) was greater than mean pre-test knowledge score of studysubjects (14.63±4.17).Mean difference of 13.75at (p value <0.001)which indicates a there is significant difference between pre-test and post– test mean knowledge scores and Overallmean post-test skillscores (18.15±1.97) wasgreaterthanmeanpre-testskillscoresofsubjects(6.52±2.51).Meandifferenceof 11.63at(pvalue<0.001)whichindicatesathereissignificant differencebetweenpre- test and post–test mean skill scores. There was no significant associationbetweenpre- test knowledgeandskill scoreswiththeirdemographicvariables(age, ,habitat,Type of family , education of parents).Conclusion:The study showed that there was a significant improvement in the knowledge scores after the administration of Self Instructional Module (SIM) and demonstration.Hence it can be concluded that the Self Instructional Module (SIM) and demonstration was effective in improving the knowledge and skillof General Nursing and Midwifery studentsregarding prevention ofHIV/AIDS.