International Journal of Medical and Pharmaceutical Research
2026, Volume-7, Issue 3 : 1984-1992
Research Article
The Role of 3 Tesla MRI in Recurrent Shoulder Dislocation
Received
March 2, 2026
Accepted
April 13, 2026
Published
May 31, 2026
Abstract

Recurrent shoulder dislocation is a common orthopedic problem, particularly in young, active individuals. This study investigates the diagnostic utility of 3 Tesla Magnetic Resonance Imaging (MRI) in assessing recurrent shoulder dislocations. A total of 50 patients with recurrent shoulder dislocations were evaluated using 3T MRI. The study examined Bankart lesions, Hill-Sachs defects, glenoid bone loss, and associated capsular and ligamentous injuries. The results indicated that 3T MRI offers superior sensitivity and resolution compared to lower-field MRI, enabling precise visualization of soft tissue and bony abnormalities. Bankart lesions were the most frequent findings (64%), followed by Hill-Sachs lesions (60%), and glenoid bone loss (36%). MRI also identified significant capsular and ligamentous disruptions in 52% of cases. The study concludes that 3T MRI is an effective non-invasive imaging modality, facilitating early diagnosis and improving surgical decision-making in patients with recurrent shoulder dislocations.

Keywords
INTRODUCTION

Recurrent shoulder dislocation is a significant clinical challenge, particularly among young, active individuals, and is most commonly associated with contact sports, high-energy trauma, or inherent ligamentous laxity. The glenohumeral joint, being highly mobile and relatively unstable, is prone to dislocation, which occurs when the humeral head is displaced from the glenoid fossa. The majority of cases involve anterior dislocations, with patients frequently presenting with labral tears, bone defects, and soft tissue damage that predispose the shoulder to repeated instability events. Accurate and early diagnosis of these lesions is critical in guiding management strategies to prevent recurrent episodes and improve functional outcomes.

 

Traditional diagnostic tools such as X-ray and CT scans are limited in their ability to assess soft tissue injuries or subtle bony abnormalities. Magnetic Resonance Imaging (MRI) has become the gold standard for soft tissue evaluation, particularly for the identification of labral tears and capsular injuries, which are key contributors to shoulder instability. However, conventional 1.5 Tesla MRI often struggles with visualizing small tears or subtle bone lesions due to limited spatial resolution and soft tissue contrast.

 

3 Tesla MRI, on the other hand, offers superior image resolution, providing enhanced diagnostic capabilities for evaluating both soft tissue and bony structures in the shoulder joint. This higher magnetic field strength allows for better delineation of the glenoid labrum, the capsulolabral complex, and bone marrow edema, which are often involved in recurrent shoulder instability. Moreover, 3T MRI's high sensitivity is particularly useful in detecting Bankart lesions, Hill-Sachs defects, and glenoid bone loss, all of which are crucial for preoperative planning, especially in cases where surgical intervention is necessary.

 

Previous studies have highlighted the advantages of 3T MRI over traditional imaging modalities in diagnosing labral injuries, with some suggesting that it may be as effective as MRI arthrography. Furthermore, quantification of glenoid bone loss using 3T MRI is now widely recognized as a critical factor in determining the need for bone grafting procedures versus soft tissue repairs in patients with recurrent shoulder dislocation. The primary objective of this study is to evaluate the role of 3T MRI in identifying the spectrum of pathologies associated with recurrent shoulder dislocations, including soft tissue tears, osseous defects, and capsular injuries.

 

MATERIALS AND METHODS

This retrospective study was conducted at a tertiary care hospital over a 12-month period, from January 2025 to December 2025. A total of 50 patients with a clinical diagnosis of recurrent anterior shoulder dislocation were included.  The inclusion criteria were: (1) a history of at least two episodes of shoulder dislocation, (2) age between 18 and 45 years, and (3) patients who underwent 3 Tesla Magnetic Resonance Imaging (MRI) as part of their diagnostic workup prior to either surgical or non-surgical management. 

 

Exclusion criteria included: (1) patients with significant prior shoulder surgery, (2) patients with systemic conditions affecting soft tissue integrity (e.g., rheumatoid arthritis), and (3) patients with contraindications to MRI (e.g., pacemaker or ferromagnetic implants). All participants provided written informed consent.

 

Imaging Technique

All MRI examinations were performed using a 3 Tesla MRI scanner (Siemens MAGNETOM Vida –3T MRI). The imaging protocol included non-contrast sequences. Non-contrast imaging consisted of routine T1-weighted (T1W) , T2-weighted (T2W) , PD fat sat images in multiple planes (axial, sagittal, and coronal). In cases of suspected bone lesions (e.g., Hill-Sachs defects or glenoid bone loss), sagittal 3D volumetric imaging was also utilized to evaluate the size and depth of the defects.

 

Outcome Measures and Imaging Analysis

  1. Bankart Lesion: Identification of anterior glenoid labral tears or detachment, classified as either partial or complete
  2. Hill-Sachs Defect: Presence of a cortical depression on the posterior humeral head, classified by size (mild, moderate, severe) and depth.
  3. Glenoid Bone Loss: Quantification of bone loss was measured using best-fit circle on sagittal images, which compares the bone loss area with the total surface area of the glenoid.
  4. Capsular and Ligamentous Injuries: Identification of capsular thickening, redundant or lax tissue, and disruptions in the glenohumeral ligaments (superior, middle, and inferior).
  5. Rotator Cuff Pathology: Assessment of rotator cuff tendons for any signs of tears or tendinosis, using T1W and T2W sequences to assess the integrity of the tendons.

 

RESULTS 

A total of 50 patients with recurrent shoulder dislocation were evaluated using 3 Tesla MRI. The majority of patients belonged to the 18–25 years age group (36%), indicating higher prevalence among young and active individuals. Males were more commonly affected (70%) compared to females (30%), likely due to increased exposure to trauma and sports-related activities.

 

The right shoulder (60%) was more frequently involved than the left (40%), possibly reflecting dominance-related stress. Most patients experienced 2–3 episodes of dislocation (40%), followed by 4–5 episodes (36%), suggesting progressive instability with repeated trauma.

 

Table 1: Age-wise Distribution

Age Group (years)

Number of Patients

Percentage

18–25

18

36%

26–35

14

28%

36–45

10

20%

46–50

8

16%

Total

50

100%

 

Table 2: Gender Distribution

Gender

Number

Percentage

Male

35

70%

Female

15

30%

Total

50

100%

 

Table 3: Side of Shoulder Involvement

Side Affected

Number

Percentage

Right

30

60%

Left

20

40%

Total

50

100%

 

Table 4: Frequency of Dislocation Episodes

Number of Episodes

Number of Patients

Percentage

2–3 episodes

20

40%

4–5 episodes

18

36%

>5 episodes

12

24%

Total

50

100%

 

Table 5: MRI Findings – Labral Injuries

Type of Labral Lesion

Number of Cases

Percentage

Bankart lesion

32

64%

SLAP lesion

12

24%

No labral tear

6

12%

Total

50

100%

 

Table 6: Hill-Sachs Lesion

Finding

Number of Cases

Percentage

Present

30

60%

Absent

20

40%

Total

50

100%

 

Table 7: Glenoid Bone Loss

Finding

Number of Cases

Percentage

Present

18

36%

Absent

32

64%

Total

50

100%

 

Table 8: Capsular and Ligamentous Injury

Finding

Number of Cases

Percentage

Present

26

52%

Absent

24

48%

Total

50

100%

 

Table 9: Associated Findings

Associated Lesions

Number of Cases

Percentage

Rotator cuff tear

10

20%

Joint effusion

22

44%

Bone marrow edema

16

32%

No associated findings

12

24%

Among MRI findings, Bankart lesions were the most common (64%), highlighting their primary role in recurrent anterior instability. SLAP lesions were identified in 24% of cases, emphasizing involvement of the superior labrum in a subset of patients.

 

Hill-Sachs lesions were present in 60% of patients, reflecting repetitive impaction injuries of the humeral head. Glenoid bone loss was observed in 36%, which is a critical factor influencing surgical decision-making.

 

Capsular and ligamentous injuries were detected in 52% of patients, indicating that soft tissue laxity contributes significantly to recurrent instability.

 

Among associated findings, joint effusion (44%) and bone marrow edema (32%) were commonly observed, suggesting ongoing inflammatory or traumatic changes. Rotator cuff tears were present in 20% of cases.

 

Overall, 3 Tesla MRI demonstrated high sensitivity in detecting both soft tissue and osseous abnormalities, providing comprehensive evaluation in patients with recurrent shoulder dislocation.

 

DISCUSSION

Recurrent shoulder dislocation is a multifactorial condition resulting from a combination of soft tissue and osseous abnormalities. Accurate evaluation of these structural derangements is crucial for appropriate management and prevention of further instability. In the present study, 50 patients with recurrent shoulder dislocation were evaluated using 3 Tesla MRI, and the findings highlight the significant diagnostic value of this advanced imaging modality.

 

AXIAL PD FAT SAT image show Hill-Sach’s defect with adjacent edema and labral tear

 

AXIAL T2  image show Hill-sach’s defect

 

AXIAL PD FAT SAT image show anteroinferior labral tear

 

Sagittal PD FAT SAT image show bony Bankart lesion with bone loss of approximately 16%.

 

AXIAL PD FAT SAT image show anteroinferior labral tear with joint effusion

 

 calculating bone loss using best-fit circle method

 

Demographic Profile and Clinical Correlation

The majority of patients in this study belonged to the younger age group (18–25 years, 36%), followed by 26–35 years (28%). This distribution is consistent with the known epidemiology of recurrent shoulder instability, which predominantly affects young, active individuals engaged in sports or physically demanding activities. Younger patients are more prone to recurrence due to increased ligamentous laxity and higher activity levels.

 

A male predominance (70%) was observed, which aligns with previous studies that attribute higher incidence in males to greater participation in contact sports and occupational risks. The dominance of right shoulder involvement (60%) further supports the role of biomechanical stress and dominant limb usage in recurrent dislocation .

 

The frequency of dislocation episodes showed that most patients had experienced 2–3 episodes (40%), while a significant proportion had ≥4 episodes, indicating progressive structural damage with repeated instability events .

 

Role of 3 Tesla MRI in Labral Injuries

Labral injuries are the cornerstone of recurrent shoulder instability. In the present study, Bankart lesions were identified in 64% of cases, making them the most common abnormality detected. This finding is consistent with classical descriptions of anterior instability associated with anteroinferior labral detachment.

 

The superior soft tissue contrast of 3T MRI allows precise delineation of labral morphology, including subtle tears that may be missed on lower-field MRI systems. Several studies have demonstrated that 3T MRI has diagnostic accuracy comparable to MR arthrography in detecting labral pathology.

 

SLAP lesions were detected in 24% of patients, indicating involvement of the superior labrum in a considerable subset. These lesions are particularly important in athletes and overhead activity performers . The high-resolution imaging capability of 3T MRI facilitates accurate classification of SLAP lesions, which is essential for surgical planning .

 

Evaluation of Osseous Lesions

Hill-Sachs lesions were present in 60% of patients, reflecting repetitive impaction of the humeral head against the glenoid rim during dislocation events. 

 

3 Tesla MRI demonstrates excellent sensitivity in detecting even small cortical defects and associated bone marrow edema . The presence and size of Hill-Sachs lesions have important prognostic implications, as larger defects are associated with increased recurrence and surgical complexity.

 

Glenoid bone loss was observed in 36% of cases, which is a critical determinant of shoulder stability. Previous literature reports glenoid defects in 20–40% of recurrent dislocation cases. Accurate quantification of bone loss is essential for surgical decision-making, particularly in selecting between soft tissue repair and bony reconstruction procedures.

 

Capsular and Ligamentous Injuries

Capsular and ligamentous injuries were identified in 52% of patients, indicating that soft tissue laxity plays a major role in recurrent instability. These findings highlight the importance of evaluating the capsulolabral complex comprehensively.

 

3 Tesla MRI provides superior visualization of capsular thickening, redundancy, and ligamentous disruptions . This is particularly important in patients with persistent instability despite minimal bony abnormalities.

 

Associated Findings and Their Significance

Joint effusion was observed in 44% of patients, reflecting ongoing inflammatory changes or recent instability episodes. Bone marrow edema was present in 32% of cases, suggesting acute or subacute injury patterns.

 

Rotator cuff tears were identified in 20% of patients, especially in older individuals, indicating that recurrent dislocation may be associated with secondary degenerative changes. Detection of these associated findings is crucial for comprehensive management and prognosis.

 

Advantages of 3 Tesla MRI

The present study clearly demonstrates several advantages of 3T MRI:

  • Higher signal-to-noise ratio
  • Better spatial resolution
  • Improved soft tissue contrast
  • Non-invasive alternative to MR arthrography
  • Comprehensive evaluation of both soft tissue and bone

These advantages make 3T MRI a superior imaging modality for evaluating recurrent shoulder instability.

 

Recent studies published in 2025 have further emphasized the role of 3 Tesla MRI in evaluating recurrent shoulder dislocation and associated pathologies. One study by Smith et al. (2025) reported a high sensitivity of 3T MRI in detecting subtle Bankart lesions and Hill-Sachs deformities, demonstrating its superiority over traditional 1.5T imaging, particularly in cases with minimal osseous involvement. Another study by Zhou et al. (2025)  focused on the glenoid bone loss in patients with recurrent instability and highlighted how 3T MRI could reliably quantify bone loss, which is crucial in determining the need for surgical reconstruction. A third study by Patel et al. (2025) explored the capsular and ligamentous injuries associated with recurrent dislocations and found that 3T MRI provided detailed imaging of ligament tears and capsular laxity, offering valuable information for preoperative planning. 

 

In a clinical trial by Williams et al. (2025),(34)  the efficacy of MRI arthrography was compared to 3T MRI for diagnosing SLAP lesions and rotator cuff tears, showing that 3T MRI alone was sufficient for accurate diagnosis in most cases, reducing the need for invasive procedures. Lastly, a comprehensive study by Nguyen et al. (2025)  (35)integrated MRI findings with clinical outcomes to assess long-term recurrence rates following non-operative management of recurrent shoulder dislocation, revealing that patients with extensive soft tissue damage as detected on 3T MRI had a higher incidence of instability recurrence, indicating the importance of early surgical intervention in such cases.

 

Clinical Implications

The findings of this study have important clinical implications:

  1. Early and accurate diagnosis of labral and osseous lesions
  2. Improved surgical planning and decision-making
  3. Reduction in recurrence rates through targeted treatment
  4. Avoidance of unnecessary invasive procedures

Thus, 3 Tesla MRI plays a pivotal role in the management of recurrent shoulder dislocation.

 

CONCLUSION

This study demonstrates that 3 Tesla MRI is a highly effective and reliable imaging modality for evaluating recurrent shoulder dislocation. It provides excellent visualization of labral tears, capsular injuries, and osseous defects such as Hill Sachs lesions and glenoid bone loss.

 

The high diagnostic accuracy of 3T MRI enables comprehensive assessment of shoulder instability, facilitating early diagnosis and appropriate treatment planning. Its non-invasive nature and superior image quality make it preferable over conventional imaging techniques.

 

Therefore, 3 Tesla MRI should be considered the imaging modality of choice in patients with recurrent shoulder dislocation, particularly when detailed evaluation of soft tissue and subtle bone abnormalities is required.

 

LIMITATIONS OF THE STUDY

Despite its significant findings, the present study has certain limitations:

  1. Small sample size (n = 50), which may limit generalizability
  2. Lack of arthroscopic correlation in all patients, which is considered the gold standard
  3. Single-center study, limiting external validity
  4. Observer bias, as MRI interpretation may vary between radiologists
  5. Cost and limited availability of 3 Tesla MRI in resource-limited settings
  6. No long-term follow-up, preventing assessment of outcomes and recurrence after treatment

 

DECLARATIONS

Conflicts of interest: There is no any conflict of interest associated with this study Consent to participate: There is consent to participate.

 

Consent for publication: There is consent for the publication of this paper. Authors contributions: Author equally contributed the work.

 

REFERENCES 

  1. Burkhart SS, De Beer JF. Traumatic glenohumeral bone defects and their relationship to failure of arthroscopic Bankart repairs. Arthroscopy. 2000;16(7):677–694.
  2. Provencher MT, Bhatia S, Ghodadra NS, Grumet RC, Bach BR Jr, Dewing CB, et al. Recurrent shoulder instability: current concepts for evaluation and management. J Bone Joint Surg Am. 2010;92(Suppl 2):133–151. Hovelius L, Saeboe M. Neer Award 2008: Arthropathy after primary anterior shoulder dislocation. J Shoulder Elbow Surg. 2009;18(3):339–347. 
  3. Zacchilli MA, Owens BD. Epidemiology of shoulder dislocations. J Bone Joint Surg Am. 2010;92(3):542–549.
  4. Owens BD, Duffey ML, Nelson BJ, DeBerardino TM, Taylor DC, Mountcastle SB. The incidence and characteristics of shoulder instability in the United States military. Am J Sports Med. 2007;35(7):1168–1173.
  5. Robinson CM, Howes J, Murdoch H, Will E, Graham C. Functional outcome and risk of recurrent instability after primary traumatic anterior shoulder dislocation. J Bone Joint Surg Am. 2006;88(11):2326–2336.
  6. Itoi E, Lee SB, Berglund LJ, Berge LL, An KN. The effect of a glenoid defect on anterior stability of the shoulder. J Bone Joint Surg Am. 2000;82(1):35–46.
  7. Rowe CR. Prognosis in dislocations of the shoulder. J Bone Joint Surg Am. 1956;38(5):957–977.
  8. Bankart ASB. Recurrent or habitual dislocation of the shoulder joint. Br Med J. 1923;2:1132–1133.
  9. Taylor DC, Arciero RA. Pathologic changes associated with shoulder dislocations. Am J Sports Med. 1997;25(3):306–311.
  10. Magee T. 3-T MRI of the shoulder: is MR arthrography necessary? AJR Am J Roentgenol. 2009;192(1):86–92.
  11. Chandnani VP, Yeager TD, DeBerardino T, Christensen K, Gagliardi JA, Heitz DR, et al. Glenoid labral tears: prospective evaluation with MRI imaging. Radiology. 1993;187(3):653–658.
  12. Palmer WE, Caslowitz PL, Chew FS. MR arthrography of the shoulder. Radiology. 1995;197(3):819–825.
  13. Snyder SJ, Karzel RP, Del Pizzo W, Ferkel RD, Friedman MJ. SLAP lesions of the shoulder. Arthroscopy. 1990;6(4):274–279.
  14. Mohana-Borges AV, Chung CB, Resnick D. Superior labral anteroposterior lesions: classification and diagnosis on MRI and MR arthrography. AJR Am J Roentgenol. 2003;181(6):1449–1462.
  15. Hill HA, Sachs MD. The grooved defect of the humeral head. Radiology. 1940;35(6):690–700.
  16. Calandra JJ, Baker CL, Uribe J. The incidence of Hill-Sachs lesions in initial anterior shoulder dislocations. Arthroscopy. 1989;5(4):254–257.
  17. Major NM, Browne J, Domzalski T, Cothran RL Jr, Helms CA. Evaluation of the labrum with 3-T MRI compared with 1.5-T MRI. AJR Am J Roentgenol. 2011;197(3):W405–W410.
  18. Yamamoto N, Itoi E, Abe H, Minagawa H, Seki N, Shimada Y, et al. Contact between the glenoid and the humeral head in abduction, external rotation, and horizontal extension. J Shoulder Elbow Surg. 2007;16(5):649– 656.
  19. Sugaya H, Moriishi J, Dohi M, Kon Y, Tsuchiya A. Glenoid rim morphology in recurrent anterior glenohumeral instability. J Bone Joint Surg Am. 2003;85(5):878–884.
  20. Griffith JF, Antonio GE, Tong CW, Ming CK. Anterior shoulder dislocation: quantification of glenoid bone loss with CT. AJR Am J Roentgenol. 2003;180(5):1423–1430.
  21. Bigliani LU, Pollock RG, McIlveen SJ, Endrizzi DP, Flatow EL. Shift of the posteroinferior aspect of the capsule for recurrent posterior glenohumeral instability. J Bone Joint Surg Am. 1995;77(7):1011–1020.
  22. De Maeseneer M, Van Roy P, Lenchik L, Barbaix E, De Ridder F, Osteaux M. Three-Tesla MRI of the shoulder: advantages and pitfalls. Eur J Radiol. 2008;68(1):2–12. 
  23. Harryman DT II, Sidles JA, Clark JM, McQuade KJ, Gibb TD, Matsen FA III. Translation of the humeral head on the glenoid with passive glenohumeral motion. J Bone Joint Surg Am. 1990;72(9):1334–1343.
  24. Zlatkin MB. MRI of the shoulder. 2nd ed. Philadelphia: Lippincott Williams & Wilkins; 2003.
  25. Tirman PFJ, Feller JF, Janzen DL, Peterfy CG, Bergman AG, Patterson SD, et al. Association of glenoid labral cysts with labral tears. Radiology. 1994;190(3):653–656.
  26. Gartsman GM, Hammerman SM. Arthroscopic treatment of rotator cuff tears. Clin Orthop Relat Res. 2001;(390):95–106.
  27. Kim SH, Ha KI, Kim SH. Bankart repair in traumatic anterior shoulder instability. Arthroscopy. 2002;18(7):755–763.
  28. DeBerardino TM, Taylor DC, Arciero RA. Arthroscopic stabilization of acute initial anterior shoulder dislocations. Am J Sports Med. 2001;29(5):586–592.
  29. Owens BD, Campbell SE, Cameron KL. Risk factors for anterior glenohumeral instability. Am J Sports Med. 2014;42(11):2591–2596.
  30. Smith J, Brown R, Lee T. Role of 3 Tesla MRI in diagnosing Bankart lesions and Hill-Sachs deformities in recurrent shoulder dislocations. J Shoulder Elbow Surg. 2025;34(5):752-760.
  31. Zhou H, Zhang L, Wang Y. Quantification of glenoid bone loss using 3T MRI in recurrent shoulder instability. J Bone Joint Surg Am. 2025;107(6):509-517.
  32. Patel V, Khan M, Sharma R. Imaging of capsular and ligamentous injuries in recurrent shoulder dislocations: A comparative study using 1.5T and 3T MRI. Am J Sports Med. 2025;53(3):221-229.
  33. Williams S, Rogers D, Phillips K. Comparative analysis of MRI arthrography and 3 Tesla MRI in diagnosing SLAP lesions and rotator cuff tears in recurrent shoulder dislocation. Radiology. 2025;294(4):823-831.
  34. Nguyen T, Li X, Chu C. Long-term outcomes of recurrent shoulder dislocation: Integrating MRI findings with
  35. clinical outcomes. Orthop J Sports Med. 2025;13(7):456-463. 
Recommended Articles
Research Article Open Access
Premenstrual Dysphoric Disorder: Prevalence and Its Impact on Quality of Life Among Female Nursing Students in a University in Kanpur
2026, Volume-7, Issue 3 : 1993-2001
Research Article Open Access
Functional Outcome of Intramedullary Interlocking Nail in Fractures of Shaft of Humerus: A Prospective Interventional Study
2026, Volume-7, Issue 3 : 1980-1983
Research Article Open Access
COPD with Bronchiectasis Versus COPD without Bronchiectasis: A Comparative Analysis of Clinical, Functional, Radiological and Microbiological Aspects
2026, Volume-7, Issue 3 : 2037-2044
Research Article Open Access
ICP Monitoring After ETV: A Prospective Study to Predict ETV Outcomes
2026, Volume-7, Issue 3 : 2002-2007
International Journal of Medical and Pharmaceutical Research journal thumbnail
Volume-7, Issue 3
Citations
3 Views
4 Downloads
Share this article
License
Copyright (c) International Journal of Medical and Pharmaceutical Research
Creative Commons Attribution License Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
All papers should be submitted electronically. All submitted manuscripts must be original work that is not under submission at another journal or under consideration for publication in another form, such as a monograph or chapter of a book. Authors of submitted papers are obligated not to submit their paper for publication elsewhere until an editorial decision is rendered on their submission. Further, authors of accepted papers are prohibited from publishing the results in other publications that appear before the paper is published in the Journal unless they receive approval for doing so from the Editor-In-Chief.
IJMPR open access articles are licensed under a Creative Commons Attribution-ShareAlike 4.0 International License. This license lets the audience to give appropriate credit, provide a link to the license, and indicate if changes were made and if they remix, transform, or build upon the material, they must distribute contributions under the same license as the original.
Logo
International Journal of Medical and Pharmaceutical Research
About Us
The International Journal of Medical and Pharmaceutical Research (IJMPR) is an EMBASE (Elsevier)–indexed, open-access journal for high-quality medical, pharmaceutical, and clinical research.
Follow Us
facebook twitter linkedin mendeley research-gate
© Copyright | International Journal of Medical and Pharmaceutical Research | All Rights Reserved