The present article describe the effect of a Astocalm plus tablet having anxiolytic effect proved by Monoamine Oxidase inhibition (MAO), Selective Serotonin Re-absorption Inhibition (SSRI), hippocampal region activation, neurite formation, GABAergic activity. The clinical validation has further proved the effect of the formulation in modifying salivary cortisol and salivary amylase in human volunteers during morning and noon hours. The herbs used in the formulation are extremely safe for oral usage. The details of the study findings and statistical significance established by U test and Sign test are presented in the article.