Background: Elevated serum uric acid levels have been associated with an increased risk of stroke. However, the prevalence of hyperuricemia in patients with acute ischemic stroke and its association with gender and age remain unclear. Materials and Methods: This retrospective study included 110 patients with acute ischemic stroke who were admitted to a single center. Serum uric acid levels were measured within 24 hours of admission. Data on age, gender, and other clinical and laboratory variables were collected from medical records. Results: The study found that 27.3% of the patients had elevated serum uric acid levels, with a mean serum uric acid level of 6.2 mg/dL. The majority of the patients had serum uric acid levels within the normal range, and only 8.2% of the patients had low serum uric acid levels. The study also found that the mean serum uric acid level was higher in males compared to females and that there was a significant association between age and serum uric acid levels, with the highest levels observed in the age group of 80 years and above. Conclusion: The present study highlights the high prevalence of hyperuricemia in patients with acute ischemic stroke, with males and older patients being at a higher risk. These findings underscore the importance of monitoring serum uric acid levels in patients with acute ischemic stroke, especially in high-risk populations. Further studies are needed to investigate the possible mechanisms underlying the association between hyperuricemia and stroke, which may provide new insights into the pathophysiology of stroke and potential therapeutic targets.