Background – Elevation in serum levels of amylase and lipase in the absence of pancreatitis, in CKD patients. Aim – Evaluation of pancreatic enzymes level in CKD patients. Materials and methods – 135 patients were included and they were classified in stage I - V of CKD according to KDIGO(Kidney Disease Improving Global Outcome) guidelines and their serum amylase and lipase levels were evaluated. Results – We included 135 patients, in the age group of 30-60 yrs. Among 135 patients, 110 patients were divided in stages from I to V of CKD (Chronic Kidney Disease) as per KDIGO (Kidney Disease Improving Global Outcome), and 25 patients were divided in Pre HD and Post HD group. The serum amylase and lipase were significantly higher in CKD patients as the stage progresses form I to V (p= 0.000). In Pre HD and Post HD group of patients mean value of serum amylase and serum lipase were significantly higher in Post HD group in comparison to Pre HD (p=0.000) Conclusion – Our study done among 135 patients indicate that there was certain amount of retention of pancreatic enzyme in CKD patients due to decreased excretion by kidneys. And patients on HD had elevated level of these enzymes in Post HD state which was probably due to increase in lipoprotein lipase from endothelial cells of capillaries and we were measuring Total lipase in serum. Secondly due to hemoconcentration which occurs during HD by removal of fluid from body. Hence levels of total amylase and lipase increases in serum after hemodialysis.