Introduction: Diabetes is a chronic disease that occurs either when the pancreas does notproduce enough insulin or when the body cannot effectively use the insulin it produces. Diabetic neuropathy is the main cause of neuropathy in the world. There is a wide range of manifestations due to involvement of various systems such as cardiovascular, gastrointestinal, genitourinary, sudomotor and neuroendocrine systems. Gall bladder involvement in diabetic autonomic neuropathy occurs in form of increased gall bladder volume & impaired gall bladder contraction. Objectives: To study regarding the autonomic neuropathy with special reference to gall bladder volume in patients with type 2 diabetes mellitus. Methodology: It is a descriptive cross sectional study conducted during February 2021 to August 2022 at VIMS Ballari. Patients fulfilling the inclusion criteria’s were enrolled in the study. A pre-tested, semi-structure questionnaire was used for data collection and statistical analysis was done to obtain the results. Results: The study included 50 patients with diabetes, with the majority being in the age group of 41-50 years. Autonomic neuropathy was observed in 36% of patients, with severe neuropathy seen in 14 patients. The duration of diabetes was found to be significantly higher in patients with autonomic neuropathy compared to those without. The mean gall bladder volume was significantly higher in diabetic patients with autonomic neuropathy compared to those without. The observed values were found to be statistically significant (p < 0.05). The study indicates a correlation between autonomic neuropathy and gall bladder volume in diabetic patients. Conclusion: In the current study we have observed a significant association between diabetes and autonomic nervous system. The gall bladder volume was significantly higher among diabetics. Hence, early detection and prompt treatment can help prevent the associated complications and morbidity related to DAN.