Introduction: Stroke is defined as rapidly developing symptoms and/or signs of focal and global loss of cerebral function lasting for at least 24 hours with no apparent cause other than of vascular origin. It constitutes a dreadful burden of disability. Modifiable risk factors include hypertension, diabetes and hypercholesterolemia, atrial fibrillation, smoking, drug abuse, and alcohol intake. Changes in the lipid profile have been suggested with formation of atherosclerosis. Aims and objective: To evaluate lipid profile levels in patient with acute ischemic stroke and to compare this parameter with severity of stroke. Materials and methods: The cross-sectional study of one and half year duration (Feb2021-Aug2022) was done on 100 patients of acute ischemic stroke presented to K.R. Hospital, Mysore. The main goal was to determine whether hypercholesterolemia is a risk factor for high severity.Lipid profile was measured by collecting the patients’ blood after fasting for 9 to 12 hours. Severity of stroke was assessed by NIHSS. Based on NIHSS scores divided into minor (1-4), moderate (5-15), moderate to severe (16- 20), severe (21-42) groups. Along with assessment with severity, lipid profile was compared between severity of stroke. Statistical analysis: Data analysed using SPSS software version 22.0 and it will be presented as descriptive statistics in form of frequency table and graphs. Association between variables will be done using chi-square test and unpaired t test for qualitative and quantitative variables. Result will be expressed as mean+/-SD. Correlation of parameters is done by Pearson’s correlation formula. A p value of <0.05 is considered statistically significant. Results and conclusion: Among 100 cases higher levels of TC, TGL, LDL were associated with more severity of stroke. 9 were in minor group, 47 were in moderate group, 29 were in moderate to severe group, 15 were in severe group. As the severity increases the mean level of TC, TGL, LDL, VLDL also increases, mean HDL level decreases. P value being <0.05, statistically Significant, showing clinical correlation with severity of stroke. Higher TC, TGL, LDL and lower HDL increases the risk of severity of stroke. Association Were strong for higher level of TC and LDL Early detection and treating with lipid lowering drugs helps to reduce the risk and prevent morbidity and mortality.