Introduction: A cover slip, also known as cover glass, is a small, thin sheet of glass that is placed over the specimen in a microscope. This is known as mounting. Mounting is the final step in histological preparation of a slide [1]. A tissue specimen must be mounted in order to improve imaging quality during microscopy and to preserve the specimen during storage [2]. The current trend is turning organic, so we tried to discover a natural Dibutyl phthalate xylene (DPX) substitute [3]. DPX is thought to be perfect, yet it has a few disadvantages [4]. The study's objective was to evaluate and compare the effectiveness of DPX as a mounting medium with sandalwood, clove, and cedarwood oils.Aim: Compare and evaluate efficacy of natural alternatives with dpx as mounting media.Materials and Procedure: From the archives, four paraffin-embedded blocks with a verified histological diagnosis were removed. From each block, three slices were removed and conventional H&E staining techniques were applied. For every group, three H&E-stained sections and three ground sections were collected. Subsequently, they underwent four distinct mounting media mounts and several parameter analyses [3].Result: The results were tabulated. The MannWhitney U test was employed to compare findings. Sandalwood oil demonstrated comparable cellular architecture to D.P.X and other oils, with statistically significant findings for various mounting mediums.Conclusion:Because of their distinct properties, sandalwood oil functioned similarly as mounting media to D.P.X. They all have a high refractive index; thus, with few modifications, they can be used a better natural alternative for mounting.