Background & objectives: Human Immunodeficiency Virus (HIV) infection causes diseases with a wide spectrum of manifestations ranging from hematological manifestations to severe opportunistic infections, and rare malignancies and it also extends to endocrinological manifestations. This study has been undertaken with the aim to dwell deeper into the endocrinological complications, which have mostly been ignored and not keenly thought of. Clinical data pertaining to Adrenal Insufficiency (AI) in HIV infection have been lacking, with a few studies in India and none in North-eastern India, where the burden of HIV infection is high. This study will provide insight into this less explored array and help clinicians who have long been treating HIV infection-related complications but seldom arrived at the endocrine foray. CD4 count was also evaluated to correlate the degree of AI with the level of CD4. Methods: In this cross-sectional study, 120 HIV-infected patients were evaluated for AI with the Short Synacthen Test. Simultaneously; CD4 levels were obtained and analyzed for the degree of AI. Results: The Prevalence of AI in this study was found to be 20%, signs of AI like hypotension, skin hyper pigmentation, and menstrual abnormalities were significant, and there was no correlation between the CD4 levels and the degree of AI. Interpretation & conclusions: This study found that AI is statistically significant between the group with AI and the unaffected group. There was no age or sex preponderance of AI and it is not associated with the duration of the disease. No correlation was found between the CD4 level and the degree of AIs.