Introduction: Present study attempts to assess the knowledge, attitude, and practices regarding hospital waste management among PG residents, nurses, and students in selected hospitals of Indore district (M.P.).Biomedical waste management has recently emerged as an issue of major concern not only to hospitals, and nursing home authorities but also to the environment. The proper management of biomedical waste has become a worldwide humanitarian topic today. It is a well-established fact that there are many adverse and harmful environmental effects including human beings caused by the ‘Hospital waste’ generated during patient care. Methodology: This study was a hospital-based cross-sectional study conducted among 150 Health care workers by simple random sampling. Pre-designed, semi-structured Observation checklist for Health care providers has been used to assess the knowledge, attitude, and practices regarding hospital waste management among PG residents, nurses, and students in selected hospitals of the Indore district. The collected data was analyzed using SPSS 21. Results- Most of the UG students and PG residents know about the color coding of BMW more than nurses. Most PG residents know that wastes in yellow bags are treated by incineration, unlike UG students and nurses. Most of the medical staff agreed that BMW reduces the incidence of hospital-acquired infection. Most of the PG residents and UG students agreed that the segregation of waste is necessary before waste disposal. Conclusion- Based on our study, PG residents had more knowledge and a better attitude toward biomedical waste management guidelines as compared to nurses and UG students.