Introduction: Ecopharmacology is the science concerned with the entry of chemicals or drugs into the environment through any route. Various factors for drug entry into environment include consumption, incorrect disposal and through effluents of pharmaceutical companies. There are guidelines for pharmaceutical waste management (like GMP and FDA guidelines) for the manufacturing units, pharmacists and consumers. There is unawareness of correct disposal methods among healthcare professionals. So this study was planned to assess the knowledge, attitude and practice (KAP) of Ecopharmacology among the health care professionals. Material and Methods: This study was a cross-sectional, survey-based study conducted to assess the knowledge, attitude, and practice of Ecopharmacology among healthcare professionals (HCPs) at a tertiary care teaching hospital in India. The data for this study were collected through a self- designed, semi-structured, pre-validated questionnaire circulated through Google forms to Health Care Professionals. Results: This study shows a knowledge gap about Ecopharmacology. The overall attitude of participants was positive. The study showed overall unsatisfactory practice among participants. Conclusion: The results showed an overall unsatisfactory practice of drug disposal and inadequate knowledge but a positive attitude towards Ecopharmacology among healthcare professionals. These findings suggests the need for training regarding hazards of improper drug disposal to address the gap in knowledge, attitude and practices towards Ecopharmacology among healthcare professionals.