Introduction: Diabetic retinopathy, most common complication of diabetes mellitus, isa microvascular disorder and ranks as the fourth major cause of blindness globally. According to the Early Treatment Diabetic Retinopathy Study (ETDRS)and Diabetic Retinopathy Study (DRS) timely intervention can reduce vision loss. Nursing and Pharmacy students serve as the resource persons for diabetic patients seeking information regarding diabetes and diabetic retinopathy. Hence, this study aimed to assess the knowledge, attitude and practices regarding diabetic retinopathy among nursing and pharmacy students in tertiary care teaching hospital. Objectives:To evaluate knowledge, attitude and practice regarding diabetic retinopathy among Nursing and Pharmacy students in tertiary care teaching hospital and to compare awareness between Nursing and Pharmacy students. Methods: This cross-sectional study was conducted among 360 Nursing and Pharmacy students by convenience sampling in a tertiary care teaching centre in Puducherry, India, from August 2023 to January 2024. Data was collected by administering the semi-structured questionnaire after taking consent and entered in Excel sheet and analysis was done using SPSS software version 23.0 Results: About 63.9% of the participants were found to be males and 36.1% were females. 52.8% were nursing students and 47.2% were pharmacy students. 62.5% of the participants had good knowledge about diabetic retinopathy and 60% had a positive attitude towards the disease. However, good practices were found in only 52.2% of the study participants. On comparison, nursing students had better knowledge, attitude and practice regarding diabetic retinopathy than pharmacy students Conclusion: According to our research, nursing and pharmacy students demonstrated a satisfactory knowlegde and positive attitude towards diabetic retinopathy. However, there is room for improvement in terms of practice level which will contribute to the early detection and treatment of diabetic retinopathy, ultimately reducing the risk of vision-threatening complications.