Objectives: The present research was conducted to evaluate the antimicrobial efficacy of herbal root canal irrigants (neem, garlic extract, Tridax procumbens) and sodium hypochlorite against Enterococcus faecalis.

Materials and Methods: Before being inoculated onto Mueller-Hinton agar plates, the E. faecalis bacterial culture was grown overnight in the brain heart infusion (BHI) broth. Antibacterial inhibition was measured using agar well diffusion. The appropriate wells in agar plates were filled with a solution of sodium hypochlorite and herbal irrigants (neem, garlic extract, T. procumbens, and A. marmelos extracts), which were then incubated for 24 hours at 37°C. The bacterial inhibition zone in each well was measured and noted. The results were tabulated and analysed using statistics.

Results: Sodium hypochlorite indicated the maximum inhibitory zone against E. faecalis, subsequently neem, garlic extract and the lowest by Tridax procumbens.

Conclusion: Neem, garlic extract, and Tridax procumbens were tested herbal remedies that demonstrated an inhibitory zone against E. faecalis. These irrigants are therefore suitable for use as root canal irrigating solutions.