Evaluating the Efficacy and Outcomes of First-Line Line Probe Assay: A Retrospective Analysis

doi:N/A

International Journal of Medical and Pharmaceutical Research

2025, Volume-6, Issue-3

Original Article

Evaluating the Efficacy and Outcomes of First-Line Line Probe Assay: A Retrospective Analysis

Dr Shivanand Gundalli

Dr Surekha Arakeri

Dr Mayuri Madhukarrao Palmate

Dr. Varsharani G. Sul

Published

June 30, 2025

Abstract

Objective: To assess the performance and demographic distribution of first-line Line Probe Assay (LPA) results among tuberculosis (TB) patients.

Design: A retrospective observational study analyzing LPA results from 183 cases over a defined time period.

Setting: Data was collected from both public and private healthcare facilities.

Participants: A total of 183 patients underwent first-line LPA testing, including 102 males and 80 females, with ages ranging from under 10 to over 71 years. HIV status, microbiological classification, and case categorization were also analyzed.

Main Outcome Measures: Distribution of cases by age, sex, HIV status, microbiological confirmation, and healthcare source; analysis of case categorization (new, retreatment, pre-MDR/MDR); and subsequent second-line LPA testing outcomes.

Results: Of the total cases, 148 were HIV non-reactive, 32 were reactive, and 3 had unknown HIV status. Most samples were obtained from public facilities (63.9%). The majority of cases were new TB cases (83.6%), with 12 retreatment cases and 3 classified as pre-MDR/MDR. Microbiological confirmation was noted in 55 cases (30%), while 110 (60%) were not confirmed. Second-line LPA testing was conducted in 50 cases, leaving 133 without second-line testing.

Conclusions: First-line LPA is an essential diagnostic tool in TB management, particularly in identifying drug resistance. However, the study highlights gaps in microbiological confirmation and the need for comprehensive data reporting to enhance TB diagnostic efficacy.

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