Background: Acute ischemic stroke (AIS) remains a leading cause of morbidity and mortality worldwide. Rapid and accurate prognostic assessment can significantly influence management and outcomes. Serum albumin and calcium are accessible biomarkers that could provide prognostic information on stroke severity and recovery.Methods: This cross-sectional study included 120 patients admitted with AIS at the Mandya Institute of Medical Sciences. Serum levels of albumin and calcium were measured upon admission and correlated with the National Institutes of Health Stroke Scale (NIHSS) scores and Barthel Index for assessing neurological severity and functional outcomes.Results: Lower serum levels of albumin and calcium were significantly associated with higher NIHSS scores at admission (p < 0.01 and p < 0.05, respectively). Patients with severe strokes had mean albumin levels of 3.2 g/dL and mean calcium levels of 7.3 mg/dL. Moreover, recovery, as indicated by improvements in NIHSS scores, showed a significant positive correlation with higher levels of albumin (correlation coefficient = 0.62, p < 0.001) and calcium (correlation coefficient = 0.59, p < 0.001).Conclusion: Serum albumin and calcium levels are significant prognostic biomarkers in AIS, correlating with the severity of stroke and recovery outcomes. Their routine assessment can aid in early prognosis and potentially guide therapeutic decisions, particularly in resource-constrained environments.