Hypomagnesemia is associated with neuropathy and abnormal platelet activity, both of which are risk factors for the emergence of DFU. This study was carried out to evaluate the effects of magnesium administration on wound healing in subjects with DFU. This randomized controlled trial was performed among 89 subjects with DFU. Subjects were divided into two groups to receive either oral magnesium oxide supplements for 4 weeks or conventional treatment. Pre- and post- intervention wound depth and appearance were scored in accordance with the SINBAD wound assessment tool. After the 4 week treatment, significant reductions in wound area and random blood sugar level in the group that did not receive Mg supplementation. A significant decrease in the wound area, and random blood sugar level, as well as better neuropathy assessment and SINBAD scores, were reported after 4 weeks in patients who received magnesium supplementation. No significant difference in patient distribution regarding the wound healing characteristics, including discharge, granulation tissue, edge sloping, surrounding normal skin, cellulitis, and exposed bone, was noted between patients receiving and not receiving Mg supplementation.