Candida species are part of the normal flora of humans. A number of variables are known to promote both superficial and deep-seated candidiasis, and they work either by disrupting the balance of the body's normal microbial flora or by reducing host resistance. Candida's pathogenicity is attributed to its virulence factors, one of which is biofilm development. The ability to produce biofilms is linked to pathogenecity and should be regarded a key virulence characteristic during candidiasis. Aim: To detect biofilm formation among the clinical isolates of Candida species using modified Tissue Culture Plate Method and antifungal susceptibility pattern in Candida species withbiofilm production. This study was a prospective study conducted during a period of 1year on the samples received in microbiology lab,Dr. S.N. Medical College, Jodhpur for culture sensitivity test.In this study,155(05.17%) Candida species strains were isolated,out of 2995 various clinical specimens.There is an increase prevalence of non Candidaalbicans 101(65.16%) species [C. tropicalis55 (35.48%), C. parapsilosis19 (12.26%), C. krusei14 (09.03%), C. and C.kefyr 04 (02.58%)] isolated from various clinical samples and showed strong biofilm producers compared to C. albicans54(34.84%) species. Out of 155 Candida strains tested 118 (76.13%) were found to be biofilm producers. The positivity was more with urine samples 77 (82.8%) followed by sputum samples 16 (72.72%) andblood 13 (50%) isolates. TCP (Tissue culture plate) method detects 40% as Strong biofilm producers (4+, 3+), 27.74% as Moderate biofilm producers (2+), 08.39% as Weak biofilm producers (1+) and 23.87% strains were biofilm negative. There was high resistance pattern among biofilm producers in comparison with non-biofilm producers. The majority of the resistance of biofilm producing isolates was belonging to fluconazole (91.43%) followed by itraconazole (86.44%), voriconazole (83.05%) and ketoconazole (71.19%).In this study, amphotericin-B was found effective against biofilm producing Candida Species. Non-biofilm producing Candida species strains were comparatively much more sensitive to these antifungal agents. Reduced susceptibility to medications like azoles, as revealed in our work, is a critical concern in the treatment of immunocompromised patients with serious illnesses. As a result, antifungal susceptibility testing and biofilm identification is a potential approach for predicting a given agent's activity in diverse clinical isolates