Introduction: Neonatal sepsis is a common cause of neonatal morbidity and mortality. Gold standard method for diagnosing neonatal sepsis is blood culture but it is time consuming, requires well equipped laboratory and trained laboratory personnel. Serum C-Reactive Protein (CRP) is an acute phase reactant, which is released in response to the inflammatory reaction. CRP levels reflect the individual's association between microbial infection. This study is conducted for evaluation of CRP role in the blood as early marker in neonatal sepsis and to find its correlation with blood culture. Methodology: This study was conducted in microbiology laboratory from Nov 2022 to Feb 2023 to evaluate the correlation of C-Reactive Protein and Blood Culture in Neonatal Sepsis. Suspected neonatal sepsis cases in the department of Pediatrics were included in the study. After taking aseptic precautions, 1-2 ml of blood was collected and inoculated into blood culture bottle containing 5 ml of Brain Heart Infusion Broth. CRP level was detected by Latex Agglutination Card test. Results: Out of total 350 samples, 246 were from female and 104 were from male. Among350 samples tested,43 were blood culture positive and 156 were CRP positive. All blood culture positive samples were CRP positive also. Among the blood culture negative samples, 113 were CRP positive. Conclusion: Early diagnosis of neonatal sepsis with the help of biomarkers like C-Reactive Protein plays a major role in reducing mortality & morbidity among the neonates.