Background: Anatomy of the coronary arteries have been widely studied due to its clinical importance. A coronary artery is defined as any artery or arterial branch that carries blood to cardiac parenchyma. Coronary arteries and their variation in branching patterns have a significant role in cardiac deaths in recent years. Objective: To study the branching patterns of human coronary vasculature and its clinical importance. Methods: This observational study was conducted at Department of Anatomy, Diabetic Association Medical College, Faridpur, Bangladesh, Faridpursadar hospital and Bangabondhu Sheikh Mujib Medical College, Faridpur, Bangladesh, from January to December 2021. A total of 30 Human heart specimens were collected to study the branching patterns of human coronary arteries and their clinical importance. The variations in branching patterns like Trifurcation, Quadrifurcation of the coronary arteries were noted in the present study. Results: The termination of left coronary artery showed a branching pattern as bifurcation in 21 (70%) specimens, Trifurcation in 5(16.7%), Quadrifurcation in 4(13.3%) out of 30 specimens in our study. We observed 30% of the variations in branching patterns of left coronary artery, whereas right coronary artery branching pattern was normal in the present study. Conclusion: Various branching patterns of coronary arteries should be acknowledged during the catheterization for coronary angiography.