INTRODUCTION: Asymptomatic bacteriuria is the significant presence of bacteria in the urine of an individual without symptoms. This study was carried out to determine the prevalence of asymptomatic bacteriuria in pregnant women attending RIMS hospital, Imphal. MATERIAL AND METHODS: The study was conducted in the Department of Microbiology, RIMS hospital from August 2017 till January 2019.A total of 380 urine samples were collected from pregnant women attending Obstetrics and Gynaecology OPD, RIMS Imphal. Culture of the urine was done on Cysteine lactose electrolyte deficient agar. For positive growth, conventional methods were performed to identify the bacteria and antibiotic susceptibility testing was done on Mueller Hinton Agar. RESULTS:Significant bacteriuria was found in 9.1% of the asymptomatic cases. 30 urine samples were contaminated and number of positive growth was 32 (17 Escherichia coli, 7 Staphylococcus saprophyticus, 4 Staphylococcus aureus, 2 Enterococcus faecium, 1 Klebsiella pneumoniae, 1 Acinetobacter baumannii. CONCLUSION: Routine urine culture should be carried out on all antenatal patients in order to identify any unsuspecting infection. This will help in reducing maternal and obstetrics complications associated with pregnancy.