Introduction: Immediate condition just after root canal therapy takes a first impression of treatment as well as clinician’s ability. In this pretext, evaluation of reduction of PEP using two different file system with Endoactivator is done. Aim: Evaluation of post endodontic pain after using Endoactivator with ProTaper Gold and ProTaper Next file system in single visit root canal treatment. Material method: A total of 100 patients were assigned who required endodontic treatment. They were divided into two groups: Group 1 (PTG-EA) and Group 2 (PTNEA). Single visit root canal was performed under local anesthesia. Endoactivator was used for final irrigation protocol in all groups. The intensity of post endodontic pain (PEP) was evaluated by Visual Analogue Scale VAS score after 6,12,24 and 48 hours. Final data was combined and statically analyzed with the help of SPSS 20.0 software at a level of being significance being 0.05. Results: As compared to ProTaper Gold, ProTaper Next showed the least score at 6 and 12 hours of PEP, there was a statistically significant difference noted. At 24 and 48 hours no statistically significance difference was noted among two groups. Conclusion: The intensity of PEP was minimum when treated with ProTaper Next file system along with Endoactivator.