Background: The spleen, a vital component of the lymphatic and immune systems, occasionally presents with congenital anomalies such as accessory spleens (splenunculi). These are ectopic nodules of splenic tissue that arise due to the failure of fusion of embryonic splenic buds. Although typically asymptomatic and discovered incidentally, accessory spleens hold significant clinical importance, especially in hematologic disorders requiring splenectomy and in radiological evaluations of abdominal masses. Objective: This study aimed to determine the incidence, anatomical location, and morphological features of accessory spleens through systematic cadaveric dissection in a tertiary medical teaching institution. Methods: A prospective observational study was conducted on 40 formalin-fixed adult human cadavers over a period of one year. The left hypochondriac region and associated peritoneal ligaments were carefully dissected. Identified nodules were examined for size, shape, vascularity, and histological features. Results: Accessory spleens were found in 2 out of 40 cadavers (5%). Both were located in typical perisplenic regions — one in the gastrosplenic ligament and the other at the splenic hilum. The nodules measured approximately 1.3 × 1.1 cm and displayed normal splenic architecture on histological examination. Conclusion: Although rare, the presence of accessory spleens must be carefully considered in anatomical, surgical, and radiological contexts. Their identification is vital to prevent misdiagnosis and ensure complete treatment in splenectomy-requiring conditions.