Introduction: Chronic Suppurative Otitis Media is one of the most notorious infective conditions, which can lead to many complications if not treated properly. It is known for its recurrence and is linked to significant morbidity.

Aims: To find out the common bacterial agents causing Chronic Suppurative Otitis Media and to determine the Antibiotic Susceptibility pattern of the isolates.

Materials & Methods: The present study was an Institutional based observational, cross-sectional study, conducted at Dept. of Microbiology in collaboration with Dept. of ENT at ICARE Institute of Medical Sciences and Research, Haldia. Total 106 patients were included in this study. Patients clinically diagnosed with CSOM are selected from the ENT Department. Pus samples from discharging ear was collected from each patients using sterile swabs which were then cultured for the bacterial flora following the standard procedures. The Antibiotic Sensitivity testing was done using the Kirby-Bauer disc diffusion method according to Clinical Laboratory Standards Institute guidelines (CLSI).

Result: In our study, 11 (10.4%) patients had No Growth, 18 (17.0%) patients had Pseudomonas aeruginosa, 12 (11.3%) patients had Pseudomonas aeruginosa + Staphylococcus aureus and 17 (16.0%) patients had Staphylococcus aureus. The result is significant at p < .05. which exhibited high resistance to Ceftazidime (CAZ) and Meropenem (MRP), suggesting a potential limitation in their efficacy. In contrast, Imipenem (IMP) demonstrated lower resistance, indicating its continued effectiveness in treatment.