Globally, arboviruses are the cause of viral encephalitis. Japanese encephalitis is common throughout India. Tamil Nadu, Andhra Pradesh, Uttar Pradesh, Bihar, Assam, West Bengal, Karnataka, Goa, and Maharashtra are among the Indian states where cases have been documented. The current study intended to determine the prevalence of Japanese encephalitis in patients at a tertiary care facility who had received a clinical diagnosis of viral encephalitis. The disease was first identified in Nagpur (1952) which is closer to chandrapur, where it was found that an unidentified viral encephalitis, later identified as JEV, a flavivirus spread by mosquitoes, had killed about 16 people. In the current study, amongst 68 samples,7 males and 2 females were tested positive. In September month the highest number of suspected cases were noted. Children aged 1 to 5 accounted for most JEV-affected individuals, and as age increased, fewer seropositive instances were reported. In places affected by the JE epidemic, immunizations' crucial role might also be cross-checked. Therefore, it may also be advantageous to include the JE Vaccine in routine vaccination programs in places where JEV is an epidemic.