Background: The prevalence of hepatitis B virus Infection in health care workers have 2-10 times greater than general population, due to their increased vulnerability to contaminated needles and syringes, as well as exposure to contaminated blood and bodily fluids, medical students are even more at risk of unintentional exposure because of their inexperience, inadequate training, and ignorance of preventative measures towards the hepatitis b infection control measure. The objective of this study is; to study of knowledge, attitude and practice of hepatitis B Infection Prevention control measure among the 2nd year MBBS and Nursing students. Methods: A cross-sectional study was conducted among 210 students from February to march 2025.  A pretested self- administered questionnaire was used to collect data regarding knowledge, attitude, and practice towards Hepatitis B infection prevention control measure. Data was gathered via non-probability convenience sampling. χ2 test was used to determine the association between categorical independent variables and categorical dependent variables. Results: Among 210 students, 77.78 % MBBS students and 76.00 % nursing students had a good knowledge towards the hepatitis B Infection control measure. The attitude and practice (76.00 % and 70.67%) are also good in nursing students compared to MBBS students in our finding. There was significant association are found with only attitude towards Hepatitis B infection control measure. Conclusion: The knowledge levels are almost good in all age group in our study and some significant differences are found in attitude and practice. These results highlight the necessity of focused educational initiatives to enhance behaviours and attitude, especially among male and MBBS students, in order to effectively prevent and control HBV infection.