Introduction: Hepatitis B virus (HBV) infection is a major global health problem affecting large number of people every year. WHO estimates that 296 million people were living with chronic hepatitis B infection in 2019, with 1.5 million new infections each year. Health-care workers and medical students in clinical years, who come in contact with the patients and their potentially infectious materials such as blood and other body fluids, are at highest risk of acquiring the infection and should be protected and they must have a proper knowledge about hepatitis B infection, modes of transmission, clinical features, complications, and preventive measures. Aims and Objectives: To assess Hepatitis B knowledge and vaccination status. Materials and Methods: A descriptive cross sectional study. Data including knowledge of Hepatitis B transmission, vaccination status was collected in a predesigned questionnaire. Results: 100% participants have taken at-least one dose of the vaccine and 78.7% (96/122) have taken all the three doses. 96.7% (118/122) and 95.1% (116/122) participants reported blood transfusion and needle prick to be one of the cause for transmission of Hepatitis B virus respectively. Conclusion: There was a significant level of awareness of hepatitis b virus transmission and vaccination status among this high risk group participants. Attitude towards getting themselves tested for serology is an important area that is good to address.