Background: Chronic osteomyelitis is a common infection especially in developing country like India. This is very common in younger children and in majority of the time it is due to hematogenous spread of bacteria. Osteomyelitis is still continued as a major problem due to treatment failure and multidrug resistance. This study was conducted to determine the pus culture of chronic osteomyelitis and their susceptibility pattern to various antimicrobial drugs.

Methods: This is prospective study in 63 patients over a period of 8 months. Cases attending orthopaedic department with chronic osteomyelitis were subjected to pus culture and sensitivity testing.

Results: Incidence of osteomyelitis in male is 73.01% and female is 26.98%. Incidence of chronic osteomyelitis following trauma is 61.90%. The most common bone involved was tibia 44.44%. The most commonly isolated organism was staph aureus 55.93 % followed by coagulase negative staph   16.95% and Pseudomonas aeruginosa 11.86 %. Staph aureus showed 100% sensitivity against Vancomycin and Linezolid.

Conclusions: Continued surveillance for incidence of drug resistance among microorganism causing chronic osteomyelitis should be done and also antimicrobial policy should be updated based on sensitivity pattern.