Background: Blood safety begins with a healthy donor. Collecting blood which is safe for transfusion is one of the principal challenges in the health care system. Transfusion of a contaminated blood increases the morbidity and mortality of the recipient. Aims and objectives: The aim of the present study was to estimate the seroprevalence of Transfusion Transmissible Infections(TTI’s) among blood donors and to estimate the prevalence of co-infection rate. Materials and methods: A retrospective study was conducted at Adichunchanagiri hospital and research centre, B.G. Nagara for a period of two years and involved testing of 3831 blood donors, who were serologically screened for HIV, HCV, HBV and Syphilis at the blood bank. Results: For a period of 2 years, a total of 3831 donors were tested. Among them 2339(61.05%) were voluntary donors and 1492(38.94%) were replacement donors. Male donors constituted for about 2681(69.98%) and females 1150(30.01%). Overall seropositivity for all TTI’s was 1.33%. Seropositivity was more among voluntary donors(0.83%) compared to replacement donors(0.5%). Seroprevalence of HIV is 0.10%, HBV is 0.96%, HCV is 0.26%, Syphilis is 0%. There were no occurrence of concomitant infections in the present study. Conclusion: Seroprevalence of HBV was more among voluntary donors, HIV and HCV seroprevalence was higher among replacement donors. Hence thorough screening of blood donors is must to prevent TTI’s to recipients. Category: Infectious diseases, Serology