Carcinoma’s of cervix remains a major killer in women in the developing world including India. According to recent data provided by the atlas of cancer in India project by the ICMR, cervical cancer is the second most common cancer in women after breast cancer .In our country , cervical cancer shows an incidence of 25 per 1,00,000 women. The association of cervical cancer with high risk HPV infection has long been established. The protein p16 serves as a surrogate marker for the oncogenic activities of HPV in replication – competent cells of cervical epithelia and its over expression is well established in CIN and invasive squamous cell carcinoma by many studies. SUMMARY: P16 can be used as a marker for determining HPV infection in Cervical intraepithelial neoplasms (CIN) and Squamous cell carcinoma (SCC) of cervix where there is non-availabitlity of HPV-PCR method in a tertiary care Hospital