Background: Moringa oleifera is most notable for its potent antioxidant properties that neutralize harmful free radicals within the body. Free radicals are produced as natural by products of metabolic processes during aging. Testosterone, a crucial hormone in male development experiences a gradual decline as men age associated with various physiological and psychological changes. One of the factors that contribute to this decline is oxidative stress which impairs the function of leydig cells that synthesize testosterone. Therefore, this study aims to identify the effectiveness of different doses of Moringa oleifera extract on testosterone levels in aged male Wistar rats.

Methods: This is an experimental study with a post-test-only control group design. The data was collected from the total samples of 27 rats and randomized into 3 groups. 9 samples of a control group, 9 samples of a group given a dose of 250mg/kg, and 9 samples of a group given a dose of 500mg/kg of Moringa oleifera extract. The extract was given through oral gavage for 21 days and blood was collected to analyze the total testosterone levels.

Results: The results of the study showed the mean value of the treatment group tends to be lower than the control group. Testosterone levels between the 3 groups obtained a p-value of 0.598 where the value was > 0.05, which means there was no significant difference between the 3 groups based on testosterone levels.

Conclusions: Moringa leaf extract was not significantly effective on testosterone levels in aging male Wistar rats. The effectiveness of Moringa leaf extract at a dose of 500mg/kg was not significantly different from a dose of 250mg/kg on testosterone levels in male Wistar rats.