Background: Probiotics are defined as “those supplements of food which contain microbial flora that has the capacity to effect human health in a beneficial way.” The most commonly used bacterial strains for probiotic preparations belong to the genera Lactobacillus and Bifidobacterium. Aim: Present study aimed to assess the knowledge, attitude, and practice of using probiotics for health among dental and medical undergraduate students of Private University, Mehsana, Gujarat.Material and Methods: Cross sectional study conducted among 2nd year dental and medical student of sankalchand Patel University to evaluate knowledge, attitude and practice of probiotic to overcome various oral health problems by filling a structured selfadministered, close-ended questionnaire including 15 questions. Informed consent will be taken from students before conducting the study. Result: 42.2% of respondents were men and 57.8% of respondents were women among the 230 undergraduate medical and dental students. According to the study, 55.2% of respondents correctly identified the response that live microorganisms are an element of probiotics, and 83.0% of participants are aware of the term probiotics. 38.3% of the participants reported never used probiotics as a medicinal supplement. 87.8% of participants said that it contributes to better dental health. Conclusions: Undergraduate medical and dental students in the current study majority of them knew that probiotics improve immunity and how well food is absorbed by the body. Still, they don't offer the additional health advantages of probiotics. There was also a bit less understanding and clarification around the phrase "prebiotic."