Background: Air pollution is a major problem of present scenario, which has serious toxicological impact on human health and environment Air pollution is considered as the major environmental risk factor in the incidence and progression of some diseases such as asthma, lung cancer. Traffic police by their profession doing well for our society. They are vulnerable people to developed morbidities related air pollution. Aims and objectives: To assess Knowledge gap of the traffic regarding morbidities, its preventive, control measures and practice of preventive and control measures related to air pollution among Traffic police in W.B (Kolkata) and to examine the association of knowledge gap with practice measures. Materials & Methods: A descriptive study was conducted “Assess knowledge gap on morbidities, its preventive, control measures and practice of using preventive measures related to air pollution among Traffic police in W.B (Kolkata).Conceptual frame work of the study was based on Pender’s health promotional model. Study was done with 200 Traffic Police under different Kolkata traffic guard, W.B. sample selection was done by probability sampling technique through lottery method. Data regarding knowledge were collected through structured questionnaire. Results: Descriptive and inferential statistics were used for data analysis. Study findings revealed that Maximum 47.5 % traffic police have good knowledge regarding morbidities, its preventive, control measures related to air pollution. Mean of the knowledge score is 6.5, median 7, mode 15.375, SD 3. There is significant association between Knowledge level of traffic police & practice of using preventive measures for prevention of morbidities regarding Air pollution among traffic police as at one degree of freedom calculated chi-square value (8.8551),yates correction (7.7944)is greater than p value at level 0.05. Conclusion: It has implication on control and prevention of occupational health hazards. Based on the study findings information booklet to be developed for increase their knowledge and practice of preventive measures during their working period