Aims and Objective: To compare the outcome between autologous blood and fibrin glue fixated conjunctival autograft in pterygium surgery. Methods and Materials: A retrospective comparative study was carried out in 40 eyes that had a primary nasal pterygium excision in tertiary hospital in South Gujarat from January 2023 to June 2023. The patients were divided into 2 groups, in Group 1 autograft was attached with autologous blood and in Group 2 with fibrin glue. Groups were compared for surgical time, postoperative discomfort and recurrence. Result: The average surgical time was least with glue i.e 37.5 min, and 45.2 min for without glue. The postoperative discomfort was minimal in both groups. No recurrence was found in both groups till 6months follow up. Conclusion: The fibrin glue is most effective method for attaching autograft with least surgical time and postoperative discomfort. Autologous blood is an effective alternative easily available, economical than glue with less surgical time and postoperative discomfort.