Background: Nursing and midwifery workforce accounts for nearly 50% of the global health workforce. Nurses play a critical role in health promotion, disease preventionand primary care. Unfortunately, few studies on the training and adherence of health personnel to hygiene recommendations have been done, despite the obvious implications forpatientsafety. Objectives:Toassesstheknowledgeofnursingstudents regarding nosocomial infections, standard precautions and hand hygiene & thenconduct training & assess the effectiveness of the intervention. Materials & Methods: A crosssectional cum interventional study with 92 nursing students were targeted for lecture on nosocomial infections, standard precautions, and hand hygiene based on the CDC and WHO guidelines. Infection Control Standardized Questionnaire ICSQwas administered as a pre and post-test. Results: Pretest score mean = 35.8 with SD = 3.95, Post test score mean = 41.52 with SD = 6.28. On applying paired T test there was statistically significant difference in pretest and post test score with p value = 0.04, t value = 8.169. Conclusion: There was significant increase in knowledge of students as a result of the intervention lecture hence repeated sessions of such trainings can help in reduction of nosocomial infections which will improve the overall quality of patient health care